JVT

Validation and Application Of A Sensitive Elisa For Vip3AA Toxin Quantification In Corn Plants Resistant To Spodoptera FrugiperdA

ABSTRACT Bacillus thuringiensis is a Gram-positive bacterium that produces toxins (e.g. Vip3Aa) that are able to induce resistance to insect plagues (e.g. Spodoptera frugiperda) in commercial crops. However, the Vip3Aa quantification in different plant tissues and phenological stages using a validated quantification assays have not been reported yet. Therefore, this study sought to validate and apply a sensitive immunoassay to quantify Vip3Aa expressed in genetically modified corn plants (GMcP). Methodologically, immunoassay validation was done following analytical technique validation regulations...

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