JVT

Use of Acclimatized Microorganisms in Validation Studies

As an expansion upon this, some microbiologists, and also some regulators, have put forward the view that at least some of the microorganisms used for method verification should be ‘acclimatized’; that is closer to the state that they are in the environment from which a test sample is drawn. Acclimatized may also be stressed or even where the organism is damaged.  The reason for considering the inclusion of such ‘stressed’ organisms in studies is because organisms that have gone through a stress response may be more difficult to recover (and, as an aside, harder to remove, inactivate...

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